A method for the extraction and quantitation of phycoerythrin from algae

A summary of a new technique for the extraction and quantitation of phycoerythrin (PHE) from algal samples is described. Results of analysis of four extracts representing three PHE types from algae including cryptomonad and cyanophyte types are presented. The method of extraction and an equation for quantitation are given. A graph showing the relationship of concentration and fluorescence units that may be used with samples fluorescing around 575-580 nm (probably dominated by cryptophytes in estuarine waters) and 560 nm (dominated by cyanophytes characteristics of the open ocean) is provided

Improved decision support for engine-in-the-loop experimental design optimization

Experimental optimization with hardware in the loop is a common procedure in engineering and has been the subject of intense development, particularly when it is applied to relatively complex combinatorial systems that are not completely understood, or where accurate modelling is not possible owing to the dimensions of the search space. A common source of difficulty arises because of the level of noise associated with experimental measurements, a combination of limited instrument precision, and extraneous factors. When a series of experiments is conducted to search for a combination of input parameters that results in a minimum or maximum response, under the imposition of noise, the underlying shape of the function being optimized can become very difficult to discern or even lost. A common methodology to support experimental search for optimal or suboptimal values is to use one of the many gradient descent methods. However, even sophisticated and proven methodologies, such as simulated annealing, can be significantly challenged in the presence of noise, since approximating the gradient at any point becomes highly unreliable. Often, experiments are accepted as a result of random noise which should be rejected, and vice versa. This is also true for other sampling techniques, including tabu and evolutionary algorithms. After the general introduction, this paper is divided into two main sections (sections 2 and 3), which are followed by the conclusion. Section 2 introduces a decision support methodology based upon response surfaces, which supplements experimental management based on a variable neighbourhood search and is shown to be highly effective in directing experiments in the presence of a significant signal-to-noise ratio and complex combinatorial functions. The methodology is developed on a three-dimensional surface with multiple local minima, a large basin of attraction, and a high signal-to-noise ratio. In section 2, the methodology is applied to an automotive combinatorial search in the laboratory, on a real-time engine-in-the-loop application. In this application, it is desired to find the maximum power output of an experimental single-cylinder spark ignition engine operating under a quasi-constant-volume operating regime. Under this regime, the piston is slowed at top dead centre to achieve combustion in close to constant volume conditions. As part of the further development of the engine to incorporate a linear generator to investigate free-piston operation, it is necessary to perform a series of experiments with combinatorial parameters. The objective is to identify the maximum power point in the least number of experiments in order to minimize costs. This test programme provides peak power data in order to achieve optimal electrical machine design. The decision support methodology is combined with standard optimization and search methods – namely gradient descent and simulated annealing – in order to study the reductions possible in experimental iterations. It is shown that the decision support methodology significantly reduces the number of experiments necessary to find the maximum power solution and thus offers a potentially significant cost saving to hardware-in-the-loop experi- mentation

Exploratory tests of a simple aero-mechanical ride comfort system for lightly loaded aircraft

Some exploratory wind tunnel and radio-controlled free-flight tests were made with a small high-wing airplane model (1.23m wing span) to study the concept of a simple aero mechanical system intended to alleviate gust loads and improve ride comfort of lightly loaded aircraft. The system consisted essentially of the outer portions of each wing being hinged in the chordwise direction and connected directly to the wing flaps using internal counter weights to provide neutral mass balance. When the wing experienced a change in velocity or angle of attack, the movable wing panels, acting as sensors and flap actuators, deflected in response to the changes in lift on the wing. The corresponding movements of the interconnected flaps tended to reduce the changes in the wing lift

Running-mass models of inflation, and their observational constraints

If the inflaton sector is described by softly broken supersymmetry, and the inflaton has unsuppressed couplings, the inflaton mass will run strongly with scale. Four types of model are possible. The prediction for the spectral index involves two parameters, while the COBE normalization involves a third, all of them calculable functions of the relevant masses and couplings. A crude estimate is made of the region of parameter space allowed by present observation.Comment: Latex file, 20 pages, 11 figures, uses epsf.sty. Comment on the observation of the spectral index scale dependence added; Fig. 3-6 improve

Gust alleviation system to improve ride comfort of light airplanes

System consists of movable auxiliary aerodynamic sensors mounted on fuselage and connected to trailing-edge flaps by rigid mechanical linkages. System achieves alleviation by reducing lift-curve slope of airplane to such a small value that gust-induced angles of attack will result in small changes in lift

High temperature glass thermal control structure and coating

A high temperature stable and solar radiation stable thermal control coating is described which is useful either as such, applied directly to a member to be protected, or applied as a coating on a re-usable surface insulation (RSI). It has a base coat layer and an overlay glass layer. The base coat layer has a high emittance, and the overlay layer is formed from discrete, but sintered together glass particles to give the overlay layer a high scattering coefficient. The resulting two-layer space and thermal control coating has an absorptivity-to-emissivity ratio of less than or equal to 0.4 at room temperature, with an emittance of 0.8 at 1200 F. It is capable of exposure to either solar radiation or temperatures as high as 2000 F without significant degradation. When used as a coating on a silica substrate to give an RSI structure, the coatings of this invention show significantly less reduction in emittance after long term convective heating and less residual strain than prior art coatings for RSI structures

Reserve requirements: A modern perspective

The discussion in many money and banking textbooks would suggest that the Federal Reserve requires depository institutions to hold a minimum level of non-interest-earning reserves because (1) reserve requirements are a monetary policy tool that allows the Fed to expand the money supply and lower interest rates, and (2) reserve requirements improve the safety and soundness of depository institutions. This article argues that this "conventional wisdom" view is too narrow. ; The Fed often uses reserve requirement changes, the authors contend, to achieve non-monetary-policy objectives, as it did in 1992 to improve the profitability of depository institutions and ease the credit crunch of that time. The authors also challenge the notion that higher reserve requirements necessarily lead to greater safety and lower default risk for depository institutions. ; The article examines the relationship between reserve requirement changes and monetary policy, with the aim of demonstrating the recent, limited usefulness of reserve requirements as a monetary policy tool. The article proposes a more modern view of reserve requirements as a tax on depository institutions, ponders who really bears this tax, and summarizes a large and growing literature suggesting that perceived bank profitability is inversely affected by announced changes in reserve requirement ratios. The article also provides new evidence that the 1992 reserve requirement reductions were not associated with an increase in default risk for financial institutions that issue reservable instruments, as the conventional view would suggest.Monetary policy ; Financial institutions ; Bank reserves